Proteome 2D-PAGE Database - Gel overview Mycobacterium tuberculosis H37Rv; broth-cultured; culture supernatant proteins

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 6_66<br> - Elongation factor EF-Tu 
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 Identified ORFs 

  

  

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  unique to M. tub H37Rv CSN, missing in BCG Copenhagen CSN

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Authors:

Max Planck Institute for Infection Biology, Core Facility Protein Analysis1, Department of Immunology2, Central Core Facility Transcriptomics3
Peter R. Jungblut1, Jens Mattow2, Ulrich E. Schaible2, Frank Siejak2, Hans-Joachim Mollenkopf3, Ursula Zimny-Arndt1, Frank Schmidt1, Stefan H.E. Kaufmann2


References:

Schmidt F, Donahoe S, Hagens K, Mattow J, Schaible UE, Kaufmann SHE, Aebersold R, Jungblut PR (2004) Mycobacterium tuberculosis: Complementary analysis of the Mycobacterium tuberculosis proteome by two-dimensional electrophoresis and isotope coded affinity tag technology. Mol Cell Proteomics 3: 24-42.

Mattow J, Schmidt F, Hohenwarter W, Siejak F, Schaible UE, Kaufmann SHE (2004) Protein identification and tracking in two-dimensional electrophoretic gels by minimal protein identifiers. Proteomics 4: 2927-2941.

Mollenkopf HJ, Grode L, Mattow J, Stein M, Knapp B, Ulmer J, Kaufmann SHE (2004) Application of Mycobacterial Proteomics to Vaccine Design: Improved Protection by Mycobacterium bovis BCG Prime-Rv3407 DNA Boost Vaccination against Tuberculosis. Infect Immun 72: 6471-6479.

Mattow J, Schaible UE, Schmidt F, Hagens K, Siejak F, Brestrich G, Haeselbarth G, Muller EC, Jungblut PR, Kaufmann SHE (2003) Comparative proteome analysis of culture supernatant proteins from virulent Mycobacterium tuberculosis H37Rv and attenuated M. bovis BCG Copenhagen. Electrophoresis 24: 3405-3420.

Schmidt F, Schmid M, Jungblut PR, Mattow J, Facius A, Pleissner K-P (2003) Iterative data analysis is the key for exhaustive analysis of peptide mass fingerprints from proteins separated by two-dimensional electrophoresis. J Am Soc Mass Spectrom 14: 943-956.

Mollenkopf H-J, Mattow J, Schaible UE, Grode L, Kaufmann SHE, Jungblut PR (2002) Mycobacterial proteomes. Methods Enzymol 358: 242-256.

Mattow J, Jungblut PR, Schaible UE, Mollenkopf H-J, Lamer S, Zimny-Arndt U, Hagens K, Müller E-C, Kaufmann SHE (2001) Identification of proteins from Mycobacterium tuberculosis missing in attenuated Mycobacterium bovis BCG strains. Electrophoresis 22: 2936-2946.

Mattow J, Jungblut PR, Müller E-C, Kaufmann SHE (2001) Identification of acidic, low molecular mass proteins of Mycobacterium tuberculosis strain H37Rv by MALDI- and ESI-mass spectrometry. Proteomics 1: 494-507.

Thiede B, Lamer S, Mattow J, Siejak F, Dimmler C, Rudel T, Jungblut PR (2000) Analysis of missed cleavage sites, tryptophan oxidation and N-terminal pyroglutamylation after in-gel tryptic digestion. Rapid Commun Mass Spectrom 14: 496-502.

Jungblut PR, Schaible UE, Mollenkopf H-J, Zimny-Arndt U, Raupach B, Mattow J, Halada P, Lamer S, Kaufmann SHE (1999) Comparative proteome analysis of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains: Towards functional genomics of microbial pathogens. Mol Microbiol 33: 1103-1117.

Mollenkopf H-J, Jungblut PR, Raupach B, Mattow J, Lamer S, Zimny-Arndt U, Schaible UE, Kaufmann SHE (1999) A dynamic bacterial 2D-PAGE database: The mycobacterial proteome via Internet. Electrophoresis 20: 2172-2180.






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