MPIforInfectionBiology-Bioinformaticsservicegroup
Bioinformatics group
Dr.K.-P.Pleissner
My working places:
• Central Institute for Molecular Biology, Berlin (1970-1991)
• Sued Laborbedarf GmbH, Gauting/Munich (1991-1992)
• German Heart Institute, Berlin (1992-1998)
• Institute of Plant Genetics and Crop Plant Research, Gatersleben (1998-2001)
• Max-Planck-Institute for Infection Biology (2001...)
My research interests:
• Digital Image Processing in Medicine and Molecular Biology
• Computer analysis of two-dimensional protein gels
• Database construction
• Software tools for bioinformatics applications
My contribution to WWW:
•HEART-2DPAGE: The Human Myocardial 2D Gel Protein Database
•RAT HEART-2DPAGE: The Rat Heart 2D Gel Protein Database
•PDBS: The Proteome Database System for Microbial Research
•Tools and databases at IPK
List of Papers:
• 1. Pleissner,K.-P., Kröber,R.:
Die Anwendung des Kleinrechnersystems KRS4200 für die on-line Erfassung und Verarbeitung von Meßdaten der Röntgen-Kleinwinkelstreuung (RKWS). Exp.Technik der Physik 24,79-86(1976)
• 2. Pleissner,K.-P., Lucius,H., Smettan,G.:
Erfassung und Verarbeitung von Meßdaten einer stopped-flow und Temperatur-Sprung-Apparatur mit dem Kleinrechnersystem KRS4200. Zeitschrift für physikalische Chemie 259,1-9(1978)
• 3. Pleissner,K.-P., Haenel,S., Trepte,P.:
Verbesserung elektronenmikroskopischer Bilder mit einem digitalen Bildverarbeitungsverfahren. Exp.Technik der Physik 26,303-307(1978)
• 4. Lutsch,G., Pleissner,K.-P., Wangermann,G., Noll,F.:
Application of a digital image processing method to the enhancement of electron micrographs of small ribosomal subunits. Acta biol.med.germ. 36,K59-K62(1977)
• 5. Pleissner, K.-P., Lutsch,G., Haenel,S., Trepte,P.:
Bildverbesserung mittels Bildakkumulation. BILD UND TON 31,311-313(1978)
• 6. Pleissner, K.-P., Lutsch, G., Wangermann, G.:
Processing of electron micrographs of ribosomal subunits by computer. studia biophysica 79,117-118(1980)
• 7. Pleissner, K.-P., Wessel,R.:
Elementare Bildpunktoperationen und Bildwiedergabe mit dem System BAG1-KRS4200. BILD UND TON 34,57-59(1981)
• 8. Wessel, R., Müller, E.-Ch., Lucius, H., Pleissner, K.-P., Kröber,R.:
Application of a microcomputer in wide angle X-ray scattering. Computer Physics Communications 26,209-211(1982)
• 9. Müller, E.-Ch., Wessel, R., Lucius, H., Pleissner, K.-P.:
Mikrorechnersteuerung eines Röntgen-Diffraktometers für die Untersuchung von Proben in Transmission. Exp.Technik der Physik 32,37-48(1984)
• 10. Pleissner, K.-P.:
Rechnergestützte Meßwerterfassung und Verarbeitung- ein Beitrag zur Automatisierung von Meßeinrichtungen der molekularbiologischen Forschung. Dissertation A, Nov.1978
• 11. Krone, J., Pleissner, K.-P., Spielberg, A.J.:
Ein multiplikatives Optimalfilter zur Bildverbesserung. BILD UND TON 37,83-86(1984)
• 12. Lucius, H., Pleissner, K.-P.:
Zur statistischen Analyse multivariater Merkmalsverteilungen in der Zytologie. BILD UND TON 37,372-374(1984)
• 13. Rudolph, M., Pleissner, K.-P.:
Application of computer-assisted image analysis for studying viral structures. Biology of Cell (France) 55,139-142(1985)
• 14. Rudolph, M., Pleissner, K.-P., Lucius, H.:
Möglichkeiten und Grenzen des Einsatzes digitaler Bildverbesserungsverfahren bei morphologischen Virusuntersuchungen Tagungsberichte der Akademie der Landwirtschaftswissenschaften der DDR 234,121-126(1985)
• 15. Pleissner, K.-P., Wessel, R., Knespel, A., Meissner, F., Behlke,J.:
Rechnergestützte Auswertung von Meßkurven der analytischen Ultrazentrifuge zur Bestimmung von Sedimemtationskoeffizienten, deren Verteilungen und Molmassen. Exp.Technik der Physik 34,139-145(1986)
• 16. Seifert, G., Pleissner, K.-P., Lucius, H., Anders, W., Beuthan, J.:
Einsatz der digitalen Bildspeicherung beim CCD-Röntgenfernsehen Feingerätetechnik 37,118-122(1988)
• 17. Pleissner,K.-P.:
Image processing of two-dimensional gels. Mathematical Research , vol. 55, Akademie-Verlag, 1989 Proc. 3rd Intern.Conference CAIP '89,p.229-236
• 18. Behlke, J., Knespel,A., Glaser, R.W., Pleissner, K.-P.:
Analysis of intra- and intermolecular interactions in some oxidases using analytical ultracentrifuge, Progress in Colloid & Polymer Science 86,30-35(1991)
• 19. M. Knecht, V. Regitz-Zagrosek, K.-P. Pleißner, S. Emig, P. Jungblut, A. Hildebrandt, E. Fleck:
Dilated Cardiomyopathy: Computer-Assisted Analysis of Endomyocardial Biopsy Protein Patterns, Eur. J. Clin. Chem. Clin. Biochem. 1994, 32, 615-624
• 20. Jungblut,P., Otto,A., Zeindl-Eberhart,E., Pleißner,K.-P., Knecht, M. Regitz-Zagrosek,V., Fleck,E., Wittmann-Liebold, B.:
Protein composition of the human heart: The construction of a myocardial two-dimensional electrophoresis database, Electrophoresis 1994, 15, 685-707.
• 21. M. Knecht, V. Regitz-Zagrosek, K.-P. Pleißner, P. Jungblut, A. Hildebrandt,E. Fleck:
Characterization of myocardial protein composition in dilated cardiomyopathy by two-dimensional gel electrophoresis, European Heart Journal 1994, 15 (supplement D), 37-44
• 22. K.-P. Pleißner, V. Regitz-Zagrosek, C. Weise, M. Neuß, B. Krüdewagen, P. Söding, K. Buchner, F. Hucho, A. Hildebrandt, E. Fleck:
Chamber-specific expression of human myocardial proteins detected by two-dimensional gel electrophoresis, Electrophoresis 1995, 16, 841-850
• 23. K.-P. Pleißner, S. Sander, H. Oswald, V. Regitz-Zagrosek, E. Fleck:
The construction of the World Wide Web-accessible myocardial two-dimensional gel electrophoresis protein database "HEART-2DPAGE": A practical approach, Electrophoresis 1996, 17, 1386-1392
• 24. K.-P. Pleißner, S. Sander, H. Oswald, V. Regitz-Zagrosek, E. Fleck:
Towards design and comparison of World Wide Web-accessible myocardial two-dimensional gel electrophoresis protein databases, Electrophoresis 1997, 18, 480-483
• 25. K.-P. Pleißner,P.Soeding, S. Sander, H. Oswald, M. Neuss, V. Regitz-Zagrosek, E. Fleck:
Dilated cardiomyopathy-associated proteins and their presentation in a World Wide Web-accessible two-dimensional gel protein database, Electrophoresis 1997, 18, 802-808
• 26. K.-P. Pleissner, L. Wernisch, H. Oswald, E. Fleck:
Representation of amino acid sequences as two-dimensional point patterns, Electrophoresis 1997, 18, 2709-2713
• 27. K.-P. Pleissner, A. Sahlstroem, S. Wegner, H. Oswald, E. Fleck:
Protein spot detection in WWW-accessible 2-D gel images using the watershed transformation, Proceedings Electrophorese Forum '97, Strassbourg Nov.1997
• 28. H. Alt, F. Hoffmann, K. Kriegel, C. Wenk, K.-P. Pleissner:
CAROL- New Algorithmic Tools for Comparing 2-D Electrophoresis Gel Images, Proceedings Electrophorese Forum '97, Strassbourg Nov.1997
• 29. Wegner, S.,Sahlstroem, A., Pleissner, K.-P., Oswald, H., Fleck, E.:
Eine hierarchische Wasserscheidentransformation f"ur die Spotdetektion in 2D Gelelektrophorese-Bildern. Bildverarbeitung in der Medizin 1998, Algorithmen-Systeme-Anwendungen, Springer Verlag, Informatik aktuell,Aachen, März 1998, pp.134-138.
• 30. Pleissner, K.-P., Regitz-Zagrosek, V., Krüdewagen, B., Trenkner, J., Hocher, B., Fleck, E.:
Effects of renovascular hypertension on myocardial protein patterns - Analysis by computer assisted 2-dimensional gel electrophoresis, Electrophoresis, 1998, 19, 2043-2050.
• 31. Pleissner, K.-P., Hoffmann, F., Kriegel, K., Wenk, C., Wegner, S., Sahlström, A., Oswald, H., Alt, H., Fleck, E.:
New algorithmic approaches to protein spot detection and pattern matching in two-dimensional electrophoresis gel databases, Electrophoresis, 1999, 20, 755-765
• 32. Li, X., Pleissner, K.-P., Regitz-Zagrosek, V., Salnikof, J., Jungblut, P.:
A two-dimensional electrophoresis database of rat heart proteins, Electrophoresis,1999, 20, 891-897.
• 33. Jungblut PR, Zimny-Arndt U, Zeindl-Eberhart E, Stulik J, Koupilova K, Pleissner KP, Otto A, Mueller EC, Sokolowska-Koehler, W, Grabher G, Stoeffler G.:
Proteomics in human disease: cancer, heart and infectious diseases. Electrophoresis,1999, 20(10), 2100-10.
• 34. Klaus-Peter Pleissner, Helmut Oswald and Susan Wegner.:
Image analysis of 2-dimensional gels, in:Proteomics - from protein sequence to function,eds.by S.R. Pennington, M.J. Dunn, BIOS ScientificPublishers Ltd, 2001
• 35. W. Michalek, W.Weschke, K.-P. Pleissner, A.Graner:
EST analysis in barley defines a unigene set comprising 4,000 genes. Theor Appl Genet (2002) 104:97-103
• 36. K.-P. Pleissner, T. Eifert, P.R. Jungblut:
A European pathogenic microorganism proteome database: construction and maintenance. Comp Funct Genom 2002;3:97-100.
• 37. Schmidt, F., Schmid, M., Jungblut, P.R., Mattow, J., Facius, A., Pleissner, K.-P.:
Iterative data analysis is the key for exhaustive analysis of peptide mass fingerprints from proteins separated by two-dimensional electrophoresis. J Am Soc Mass Spectrom 2003 Sep; 14(9):943-56.
• 38. K.-P. Pleissner, T. Eifert, S. Buettner, F. Schmidt, M. Boehme, T.F. Meyer, S.H.E Kaufmann, P.R. Jungblut:
Web-accessible proteome databases for microbial research. Proteomics, 2004, 4, 1305-1313.
• 39. Krah, A., Miehlke, S., Pleissner, K.-P., Zimny-Arndt, U., Kirsch, C., Lehn, N., Meyer, T.F., Jungblut, P.R., Aebischer T.:
Identification of candidate antigens for serologic detection of Helicobacter pylori-infected patients with gastric carcinoma. Int J Cancer 2004, 108(3),456-463.
• 40. Krah, A., Wessel, R., Pleissner, K.-P.:
Assessment of Protein Spot Components Applying Correspondence Analysis for Peptide Mass Fingerprint Data. Proteomics 2004, 4(10):2982-6.
• 41. Pleissner, K.-P., Schmelzer, P., Wehrl, W., Jungblut, P.R.:
Presentation of differentially regulated proteins within a web-accessible proteome database system of microorganisms Proteomics 2004, 4(10):2987-90.
• 42. Gressmann, H., Linz, B., Ghai, R., Pleissner, K.-P., Schlapbach,R., Yamaoka, Y., Kraft, C., Suerbaum, S., Meyer, T.F., Achtman, M.:
Gain and Loss of Multiple Genes During the Evolution of Helicobacter pylori. PLoS Genet. 2005 Oct 7; 1(4):e43
• 43. Paape, D., Lippuner, C., Schmid, M., Ackermann, R., Barrios-Llerena, Martin E., Zimny-Arndt, U., Brinkmann, V., Arndt, B., Pleissner, K.-P., Jungblut, P.R., Aebischer, T:
Transgenic, Fluorescent Leishmania mexicana Allow Direct Analysis of the Proteome of Intracellular Amastigotes. Molecular & Cellular Proteomics 7:1699-1701, 2008.
• 44. Hoehenwarter, W., Tang, Y., Ackermann, R., Pleissner, K.-P., Schmid, M., Stein, R., Zimny-Arndt, U., Kumar, N.M., Jungblut, P.R.
Identification of proteins that modify cataract of mouse eye lens. Proteomics. 2008 Dec;8(23-24):5011-24.
• 45. Schmidt, F., Schmid, M., Thiede, B., Pleissner, K.-P., Boehme, M., Jungblut, P.R.
Assembling proteomics data as a prerequisite for the analysis of large scale experiments.Chem Cent J. 2009 Jan 23;3(1):2.